目的:研究脑康Ⅱ号对糖尿病大鼠学习记忆功能的干预情况。方法:将3月龄雄性Wistar大鼠分为对照组、模型组、脑康Ⅱ号干预组(分为高、中、低剂量3组),模型组和脑康Ⅱ号干预组用链脲佐菌素60 mg·kg-1剂量ip,对照组ip相同体积的枸橼酸缓冲液,脑康Ⅱ号干预组分别使用20,10,5 g·kg-1的脑康Ⅱ号ig,采用水迷宫实验和气相色谱-质谱联用仪,分别观察糖尿病大鼠的行为学改变和血清3-脱氧葡糖醛酮(3-DG)的变化。结果:造模后模型组和中药干预组大鼠血糖较对照组显著升高(P<0.01)。Morris水迷宫检测大鼠到达站台时间显示,模型组需(18.6±6.3)s时间较对照组(7.5±3.1)s明显延长,具有显著性差异(P<0.01)。脑康Ⅱ号各治疗组所需时间(低、中、高剂量组分别为(9.9±3.2)s,(8.7±4.1)s,(10.1±3.9)s,较模型组明显减少(P<0.01)。糖尿病大鼠模型血清中3-DG含量(20.19±2.62)ng.mL-1,较对照组显著升高(P<0.05),约为对照组(7.02±1.19)ng.mL-1的2.5倍。脑康Ⅱ号高剂量治疗组3-DG含量较模型组显著降低(8.89±1.09)ng.mL-1(P<0.05)。结论:脑康Ⅱ号能够通过降低大鼠血清3-脱氧葡糖醛酮的水平,改善糖尿病大鼠认知障碍。 Objective: To study the effect of Naokang Ⅱ on learning and memory in diabetic rats. Methods: Three-month-old male Wistar rats were divided into control group, model group and Naokang Ⅱ intervention group (divided into high, middle and low dose groups). The model group and the Naokang Ⅱ intervention group were given streptozotocin 60 mg · kg-1 of ciclosporin ip, the same volume of citrate buffer in control group ip, and No.2 of Nao Kang Ⅱ group were treated with 20, 10, 5 g · kg-1 of igang Ⅱ, Maze experiment and gas chromatography-mass spectrometry were used to observe the changes of behavior and the changes of serum 3-deoxyglucosone (3-DG) in diabetic rats. Results: Compared with the control group, the blood glucose in model group and traditional Chinese medicine intervention group were significantly increased (P <0.01). Morris water maze test to reach the platform time showed that the model group was (18.6 ± 6.3) s significantly longer than the control group (7.5 ± 3.1) s, with significant difference (P <0.01). The time required for treatment of Naokang Ⅱ was (9.9 ± 3.2) s, (8.7 ± 4.1) s and (10.1 ± 3.9) s respectively, which was significantly lower than that of model group (P <0.01) The level of 3-DG (20.19 ± 2.62 ng · mL-1) in the serum of diabetic rats was significantly higher than that of the control group (P <0.05), which was about 7.02 ± 1.19 ng · mL-1 2.5-fold.The content of 3-DG in high-dose Naokang Ⅱ group was significantly lower than that in model group by 8.89 ± 1.09 ng.mL-1 (P <0.05) .Conclusion: Deoxyglucosone levels improve cognitive impairment in diabetic rats.