论文部分内容阅读
[目的]建立测定动物胃肠内压实验模型,为研究药物调节动物胃肠运动提供简便而有效的方法。[方法]参考大鼠离体胃肌条运动方法、水囊灌注测压法和豚鼠回肠蠕动实验,建立灌注式大(豚)鼠离体胃肠内压测定模型,观察大(豚)鼠离体胃肠腔内压力变化情况。[结果]①营养液中加入1.25,2.5,3.25,5,6.25,7,5μg/mL剂量乙酰胆碱的大(豚)鼠腔内压明显增高,与蒸馏水组比较,P<0.01～0.001。②营养液中加入0.125,0.25,0.5μg/mL剂量的组胺的豚鼠离体肠内净增压增高,与加0.4mL蒸馏水的蒸馏水组比较,P<0.01～0.01。③加入250,500,1000,2000μg/mL四君子汤正丁醇部位,使大鼠离体胃运动受到抑制,与蒸馏水组比较,P<0.05～0.001。④加入125,250,500,1000,2 000 μg/mL四君子汤正丁醇部位,小剂量(125 μg/mL)对豚鼠回肠有轻微兴奋作用,大剂量(250～2000μg/mL)使豚鼠回肠运动受到抑制,与蒸馏水组比较,P<0.05～0.001。[结论]不同浓度的乙酰胆碱能使大鼠离体胃内压升高,而组织胺能使豚鼠离体回肠内压升高,说明胃肠运动对药物刺激反应良好,此模型可用于药理学实验研究。四君子汤正丁醇部位具有抑制胃肠运动的作用。
[Objective] To establish an experimental model for determining the gastrointestinal pressure in animals and provide a simple and effective method for studying the regulation of drugs on gastrointestinal motility in animals. [Method] With reference to the method of gastric muscle strips movement in vitro, the hydronephrostatic method and the ileal peristalsis test in guinea pigs, the model of peritoneal dialysis of perfused large dolphin was established. Gastrointestinal cavity pressure changes. [Result] ① The intraluminal pressure of rats fed with 1.25, 2.5, 3.25, 5, 6.25, 7 and 5μg / mL acetylcholine significantly increased, P <0.01-0.001 compared with distilled water group. ② Enteral administration of 0.125,0.25,0.5μg / mL histamine in the nutrient solution increased intestinal net pressure, compared with 0.4ml distilled water distilled water group, P <0.01 ~ 0.01. ③ Adding 250, 500, 1000, 2000μg / mL n-butanol fraction of Sijunzi Decoction inhibited gastric motility in rats, compared with distilled water group, P <0.05 ~ 0.001. ④ The ileum of guinea pig ileum was slightly excited by 125, 250, 500, 1000 and 2000 microg / mL Sijunzi decoction, and the guinea pig ileum was inhibited by high dose (250-2000 μg / mL) Group comparison, P <0.05 ~ 0.001. [Conclusion] Different concentrations of acetylcholine can increase the gastric pressure in isolated rat, while histamine can increase the ileum pressure of isolated guinea pig, indicating that gastrointestinal motility is good for drug stimulation. This model can be used in pharmacological experiments the study. Sijunzi Tang nbutanol site has the effect of inhibiting gastrointestinal motility.