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目的探讨经福尔马林固定1d石蜡包埋组织(FFPET)提取DNA的简易有效方法。方法比较水浴加热、微波加热和二甲苯脱蜡的效果。组织脱蜡后分别采用Chelex-100+层析柱纯化法、DNA IQTM试剂盒磁珠提取法和Chelex-100+磁珠纯化法提取DNA;实时荧光定量PCR技术定量DNA;荧光标记毛细管电泳技术进行STR分型。结果二甲苯脱蜡的效果好于其他两种加热的脱蜡方法(P<0.05)。Chelex-100+层析柱纯化所获得的DNA量显著高于其他两种方法(P<0.05)。结论二甲苯脱蜡、Chelex-100+层析柱纯化法是一种简单、有效的FFPET处理方法。
Objective To investigate a simple and effective method for DNA extraction from formalin-fixed 1d paraffin-embedded tissue (FFPET). Methods The effects of water bath heating, microwave heating and xylene dewaxing were compared. Tissues were deparaffinized using Chelex-100 + column purification method, DNA IQTM kit magnetic beads extraction and Chelex-100 + magnetic beads purification DNA; quantitative real-time PCR quantitative DNA; fluorescence-labeled capillary electrophoresis STR typing. Results The dewaxing of xylene was better than the other two dewaxing methods (P <0.05). The amount of DNA obtained from Chelex-100 + column purification was significantly higher than the other two methods (P <0.05). Conclusion Xylene dewaxing, Chelex-100 + column purification method is a simple and effective FFPET treatment.