目前制备单克隆抗体多用标准型自动二氧化碳培养箱,或以干燥缸内产CO_2代替。作者以普通培养箱改装成简易CO_2培养箱,取代昂贵的CO_2孵箱,并克服了干燥缸容积有限,操作繁杂的不足,用于杂交瘤细胞的培养及建株研究,效果满意。 The current preparation of monoclonal antibodies and more use of standard automatic carbon dioxide incubator, or to replace the cylinder produced CO_2. The author changed into a simple CO 2 incubator with an ordinary incubator instead of an expensive CO 2 incubator and overcame the limitations of the dry cylinder volume and complicated operation for the study of hybridoma cell culture and plant construction with satisfactory results.