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目的:观察痛泻要方对内脏高敏感大鼠的影响,初步探讨其作用机制。方法:30只SD幼鼠随机分为正常组、模型组和中药组。采用母婴分离联合束缚应激的方法建立腹泻型肠易激综合征(IBS-D)大鼠内脏高敏感模型。正常组、模型组予0.9%氯化钠溶液,中药组予痛泻要方煎剂灌胃。给药前后,观察3组大鼠粪便Bristol分级评分、粪便含水量及腹壁撤退反射(AWR);采用Real-Time PCR法检测结肠CFTR Cl-通道蛋白表达。结果:模型组粪便Bristol评分、含水量均高于正常组,内脏痛阈值下降(P<0.01);给药后,中药组Bristol评分及含水量下降(P<0.01),内脏疼痛阈值升高(P<0.01);与正常组及模型组比较,中药组结肠CFTR Cl-通道蛋白表达水平下降(P<0.05)。结论:痛泻要方可以降低IBS-D大鼠内脏敏感性,机制可能是通过影响结肠上皮的CFTR Cl-通道蛋白表达。
Objective: To observe the effect of Tongxieyaofang on visceral hypersensitivity in rats and to explore its mechanism. Methods: Thirty SD young rats were randomly divided into normal group, model group and TCM group. To establish the model of visceral hypersensitivity in rats with diarrhea-predominant irritable bowel syndrome (IBS-D) by using the method of separation of maternal-fetal separation and restraint stress. The normal group, the model group to 0.9% sodium chloride solution, the Chinese medicine group Tongyao Yao Fang decoction gavage. Before and after administration, the stool Bristol grading score, stool water content and abdominal wall reflex (AWR) were observed in 3 groups. Real-time PCR method was used to detect the colon CFTR Cl-channel protein expression. Results: The Bristol score and the water content in the model group were higher than those in the normal group and the thresholds of visceral pain were decreased (P <0.01). After administration, the Bristol score and the water content decreased (P <0.01) and the visceral pain threshold increased P <0.01). Compared with the normal group and model group, the expression of CFTR Cl-channel protein in the colon of the TCM group decreased (P <0.05). Conclusion: Tong Xie Yao Fang can reduce visceral sensitivity in IBS-D rats, which may be through the influence of CFTR Cl-channel protein expression in the colon epithelium.