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应用培养角膜内皮细胞的方法,是研究正常及病变角膜内皮细胞形态及机能的重要手段之一。本文为了解培养的正常家兔角膜内皮细胞形态学,为进一步施加各种处理因素提供对照,我们于1986年以培养的家兔角膜内皮细胞为材料在倒置相差显微镜及扫描电镜下,对其形态进行了观察,现报告如下。材料及方法一、实验动物及取材方法:白色家兔,体重2.0～2.5kg,处死后立即摘除眼球,用PBS溶液洗净眼球后,在实体显微镜下,
The method of culturing corneal endothelial cells is one of the important means to study the morphology and function of normal and diseased corneal endothelial cells. In order to understand the morphology of cultured normal rabbit corneal endothelial cells, to provide further control for various treatment factors, we cultured rabbit corneal endothelial cells in 1986 under the inverted phase contrast microscope and scanning electron microscope, its morphology Observed, the report is as follows. Materials and methods First, the experimental animals and access methods: white rabbits, weighing 2.0 ~ 2.5kg, immediately after the removal of the eyeballs, washed with PBS solution eyeballs, under a solid microscope,