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运用体外实验、对Vc／FeSO_4。诱发的微粒体脂质过氧化（LP）发生条件及反应历程进行了探讨。结果表明：在37℃水浴30min，微粒体蛋白浓度为1.0mg／ml，4μmol／LFeSO_4.pH值7．4的0．1mol／LTris－HCl缓冲浪的条件下，Vc低浓度（0.25mmol/L以下）时可刺激LP，但高浓度的Vc（＞0.25mmol/L）则抑制LP，其中Vc为0．05mmol/L时．微粒体中MDA含量于反应后迅速上升，30min达到高峰.以后趋于平稳。揭示：一定比例的Vc／FeSO_4可有效地激发微粒体LP。本研究初步建立了微粒体LP模型。
In vitro experiments, the Vc / FeSO_4. Induced microsomal lipid peroxidation (LP) the occurrence of conditions and reaction process were discussed. The results showed that under the conditions of 37 ℃ water bath for 30min, microsomal protein concentration of 1.0mg / ml, 4mol / L FeSO_4.pH value 7.4 of 0.1mol / L Tris-HCl buffer wave, Vc low concentration (0.25mmol / L LP), but high concentration of Vc (> 0.25 mmol / L) inhibited LP, with Vc at 0.05 mmol / L. The content of MDA in microsome increased rapidly after the reaction, reached the peak at 30min, then stabilized. It is revealed that a certain proportion of Vc / FeSO 4 can effectively stimulate microsomal LP. In this study, a microscopic LP model was established.