论文部分内容阅读
西班牙科学家发现,通过特异性抑制SMAD-2/3信号通路,能从人类胚胎干细胞(human embryonic stem cells,hESCs)中有效地富集间充质干细胞(mesenchymal stem cells,MSCs)。通过此法获得的人类胚胎干细胞源性间充质干细胞(hESC-MSCs)形似成纤维细胞,表达MSCs的表型,即CD73~+ CD90~+ CD105~+ CD44~+ CD166~+ CD45~- CD34~- CD14~- CD19~- HLADR~-。对hESCs进行抑制SMAD-2/3信号通路的处理,培养28天后,培养体系中具有多能性的MSCs数量显著增加(>42%)。应用荧光激活细胞分选法(FACS)分离得到CD73~+ CD90~+ hESC-MSCs,建立该细胞系并对其长期培养。在维持MSCs形态和表型的前提下,hESC-MSCs的生长速度均比体细胞源性MSCs快。此外,在体外培养和体内实验中,这些细胞
Spanish scientists found that mesenchymal stem cells (MSCs) can be efficiently enriched from human embryonic stem cells (hESCs) by specifically inhibiting the SMAD-2/3 signaling pathway. Human embryonic stem cell-derived mesenchymal stem cells (hESC-MSCs) obtained by this method are similar to fibroblasts and express the phenotypes of MSCs, that is, CD73 ~ + CD90 ~ + CD105 ~ + CD44 ~ + CD166 ~ + CD45 ~ - CD34 ~ - CD14 ~ - CD19 ~ - HLADR ~ -. Treatment of hESCs with SMAD-2/3 signaling pathway resulted in a significant increase (> 42%) of pluripotent MSCs in culture system after 28 days of culture. CD73 ~ + CD90 ~ + hESC-MSCs were isolated by fluorescence activated cell sorting (FACS), and the cell lines were established and cultured for a long time. Under the premise of maintaining the morphology and phenotype of MSCs, hESC-MSCs grow faster than somatic cell-derived MSCs. In addition, these cells are cultured in vitro and in vivo