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目的 :观察复方 86 1对四氯化碳大鼠肝纤维化模型Ⅰ、Ⅲ型胶原mRNA表达的影响。方法 :实验分为对照组 (2 0只 )、四氯化碳模型组 (2 0只 )、复方 86 1干预组 (2 0只 ) 3组 ,实验 8周 ,处死大鼠 ,取肝组织 ,应用RT -PCR检测肝组织Ⅰ型胶原、Ⅲ型胶原表达和病理组织特殊染色。结果 :表明与模型组 (0 .90± 0 .0 3、0 .9± 0 .0 5 )比较 ,复方 86 1组Ⅰ、Ⅲ型胶原mRNA水平分别为 0 .77± 0 .0 6、0 .75± 0 .0 5 ,差异具有显著性 (P <0 .0 1)。结论 :复方中药 86 1能够抑制Ⅰ、Ⅱ型胶原的基因水平 ,减少细胞外基质的沉积 ,是其抗肝纤维化的作用机理之一。
Objective : To observe the effect of compound 86 1 on mRNA expression of type I and type III collagen in rat models of hepatic fibrosis induced by carbon tetrachloride. Methods: The experiment was divided into three groups: control group (20), carbon tetrachloride model group (20), and compound 86 1 intervention group (20). Eight weeks after the experiment, the rats were sacrificed and the liver tissue was taken. RT-PCR was used to detect the expression of type I collagen and type III collagen in liver tissue and the special pathological tissue staining. Results: Compared with the model group (0.90±0.30, 0.9±0.05), the mRNA levels of type I and III collagen in the compound 86 1 group were 0.77±6.0 and 0, respectively. The difference was significant (P < 0.01). .75 ± 0.05. Conclusion : Compound Chinese herbal medicine 86 1 can inhibit the gene level of type I and type II collagen and reduce the deposition of extracellular matrix, which is one of the mechanisms of anti-hepatic fibrosis.