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目的探讨两起相距210多公里乡镇同期发生的肠炎沙门菌食物中毒分离株之间的分子流行病学关系。方法将两起食物中毒事件患者和剩余食物分离到的8株肠炎沙门菌进一步鉴定培养,挑取单个菌落增菌,用限制性内切酶Xba I消化酶切后进行脉冲场凝胶电泳,获得的分子分型指纹图谱,运用Bionumerics 5.1进行聚类分析。结果指纹图谱聚类显示8株肠炎沙门菌分为两个分子型,D乡食物中毒分离到的5株肠炎沙门菌有相同的指纹图谱;P镇的3株分离株图谱也一致;两起食物中毒分离株图谱之间有4个条带的差异。结论两起食物中毒的暴发虽然都是肠炎沙门菌引起的,但具有不同的分子型别,食物中毒不是由同一种污染的食物源引起;脉冲场凝胶电泳可有效应用于食物中毒溯源分析及肠炎沙门菌分子流行病学研究。
Objective To investigate the molecular epidemiological relationship between Salmonella enteritidis food poisoning isolates from two villages over 210 kilometers away from each other. Methods Eight strains of S. Enteritidis isolated from patients with food poisoning and the remaining food were further identified and cultivated. Single colonies were selected for enrichment and digested with restriction endonuclease Xba I for pulse-field gel electrophoresis to obtain Molecular typing fingerprinting, cluster analysis using Bionumerics 5.1. Results The fingerprinting cluster analysis showed that the eight strains of S. Enteritidis were divided into two molecular types. The five strains of S. Enteritidis isolated from D-type food poisoning had the same fingerprints; There are four bands between the poisoning isolates. Conclusions Both outbreaks of food poisoning are caused by Salmonella enteritidis, but have different molecular types. Food poisoning is not caused by the same kind of contaminated food source. Pulsed-field gel electrophoresis can be effectively applied to traceability analysis of food poisoning and Molecular epidemiology of Salmonella enteritidis.