在转基因小鼠的制备中,对于所导入外源DNA的检测一般采用DNA-DNA分子杂交法.由于它具有敏感性和可靠性高等优点,故它在转基因小鼠的研究中是不可少的.然而,这种方法在实际应用中也有一些缺点,如操作复杂、工作量大、实验周期长、DNA样品量大以及放射性同位素来源的时间限制等,这些都给转基因小鼠的制备带来了一定的困难.我们在人凝血IX因子(Clotting factor IX)转基因小鼠的制备中,将多聚酶链式反应(Polymerase chainreaction,PCR)技术应用于转基因小鼠的初筛,并与Southern杂交结果进行了比较.结果表明这种方法是可行的,其最大的优点是提高了效率,缩短了实验周期. In the preparation of transgenic mice, DNA-DNA hybridization is generally used for the detection of introduced foreign DNA, which is essential for the study of transgenic mice due to its high sensitivity and reliability. However, this method has some disadvantages in practical application, such as complicated operation, heavy workload, long experimental period, large amount of DNA sample, and time limit of radioisotope source, all of which bring a certain amount of preparation to transgenic mice We have applied Polymerase chain reaction (PCR) technique to the primary screening of transgenic mice in the preparation of clotting factor IX transgenic mice and compared them with the result of Southern hybridization The results show that this method is feasible, and its biggest advantage is to improve the efficiency and shorten the experimental period.