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目的:评价外阴鳞癌中PTEN和CPP32(Caspase-3)蛋白的表达与临床意义及相互关系。方法:用免疫组化S-P法对30例外阴鳞癌和10例外阴正常皮肤石蜡标本进行检测。结果:外阴鳞癌PTEN及CPP32蛋白阳性率分别为56.7%和53.3%。两者在分化好的外阴鳞癌中均较强表达,在分化差的外阴鳞癌中表达降低。PTEN的表达与外阴鳞癌临床分期、淋巴结转移有关(P<0.05);CPP32的表达与外阴鳞癌临床分期及淋巴结转移无关(P>0.05)。外阴鳞癌中PTEN与CPP32的表达呈正相关。结论:PTEN蛋白和CPP32在外阴鳞癌组织中表达下调,提示PTEN可能通过抑制CPP32表达,阻止细胞凋亡,参与细胞癌变过程,进而影响外阴鳞癌临床生物学行为。
Objective: To evaluate the expression of PTEN and CPP32 (Caspase-3) in vulvar squamous cell carcinoma and its clinical significance and correlation. Methods: 30 cases of vulvar squamous cell carcinoma and 10 cases of vulvar normal skin paraffin were detected by immunohistochemical S-P method. Results: The positive rates of PTEN and CPP32 in vulvar squamous cell carcinoma were 56.7% and 53.3% respectively. Both differentiated vulvar squamous cell carcinoma are strongly expressed in poorly differentiated vulvar squamous cell carcinoma expression decreased. The expression of PTEN was correlated with the clinical stages and lymph node metastasis of vulvar squamous cell carcinoma (P <0.05). The expression of PTEN was not related to the clinical stage and lymph node metastasis of vulvar squamous cell carcinoma (P> 0.05). The expression of PTEN and CPP32 in vulvar squamous cell carcinoma was positively correlated. Conclusions: The down-regulation of PTEN protein and CPP32 in vulvar squamous cell carcinoma suggests that PTEN may be involved in the process of cell carcinogenesis through inhibiting the expression of CPP32, preventing the apoptosis of cells and further affecting the clinical biological behavior of vulvar squamous cell carcinoma.