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目的:采用UPLC法测定地黄中梓醇的含量。方法:色谱柱ACQUITY UPLC BEH RP18(2.1mm×100mm,1.7μm);流动相为乙腈-水(5∶95);检测波长为210nm;流速为0.08mL.min-1;柱温为35℃。结果:梓醇与其他峰均能达到基线分离;其色谱峰形好。梓醇的含量在(3.06～18.36)μg时与峰面积呈良好的线性关系(Y=4417.4x+860.53,R=0.9997),平均回收率为100.97%,方法精密度RSD=1.56%(n=6)。结论:该方法简便、快速、准确、重复性好,可作为地黄中梓醇的有效检测方法。
Objective: To determine the content of catalpol in Radix Rehmanniae by UPLC. METHODS: The column was ACQUITY UPLC BEH RP18 (2.1 mm × 100 mm, 1.7 μm). The mobile phase was acetonitrile-water (5:95). The detection wavelength was 210 nm. The flow rate was 0.08 mL.min-1. The column temperature was 35 ℃. Results: Catalpol and other peaks were able to achieve baseline separation; its peak shape was good. The concentration of catalpol in the range of 3.06 ~ 18.36 μg showed a good linear relationship with the peak area (Y = 4417.4x + 860.53, R = 0.9997). The average recovery was 100.97%. The precision was RSD 1.56% 6). Conclusion: The method is simple, rapid, accurate and reproducible. It can be used as an effective method for the determination of catalpol in Rehmannia glutinosa.