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用电子自旋共振技术和化学发光(CL)法研究了川芎嗪(Ligustrazinge,Lig)对活性氧自由基的清除作用。Lig25ml~(-1)对两种水溶液体系产生的O_2~-和OH-的清除率分别为100%和44%。该作用被CL证实。Ligmg25mg.ml~(-1)对一种脂溶液体系产生的LOO的清除达80%。提示Lig能直接清除具细胞毒性作用的自由基(O_2~-,LOO,OH-)。
Electron spin resonance and chemiluminescence (CL) were used to study the scavenging effect of Ligustrazinge (Lig) on reactive oxygen species. The removal efficiencies of O_2 ~ - and OH ~ by Lig25ml ~ (-1) to both aqueous solutions were 100% and 44%, respectively. This effect is confirmed by the CL. Ligmg 25mg. ml ~ (-1) of a lipid solution generated LOO clearance up to 80%. Tip Lig can directly remove the cytotoxic effects of free radicals (O2 -, LOO, OH-).