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目的观察慢性苯并芘染毒大鼠对记忆及海马组织中谷氨酸盐受体基因(AMPA1)表达影响。方法将36只雄性SD大鼠分为高、中、低剂量组及溶剂对照组,每组9只;染毒结束后,用Morris水迷宫测试大鼠空间学习记忆能力,基因芯片技术进行AMPA1基因表达谱分析,再用逆转录-实时荧光定量PCR技术测量大鼠海马组织中AMPA1基因表达状况。结果第5 d定位航行试验中,与对照组(6.50±2.57)s比较,中、高剂量组寻找平台平均潜伏期(17.90±7.71)、(16.20±4.74)s,明显延长(P<0.05);基因芯片扫描提示AMPA1 mRNA在暴露组中表达量是对照组的2.052倍,荧光定量PCR检测显示AMPA1mRNA上调4.257倍。结论苯并芘影响大鼠的空间学习记忆能力,且明显诱导海马组织内的AMPA1基因高表达。
Objective To investigate the effect of chronic benzopyrene exposure on the expression of glutamate receptors (AMPA1) in memory and hippocampus of rats. Methods Thirty-six male Sprague-Dawley rats were divided into high, medium and low dose groups and solvent control group, with 9 rats in each group. After the exposure, the spatial learning and memory ability of rats was tested by Morris water maze and the AMPA1 gene The expression profile of AMPA1 in rat hippocampus was detected by reverse transcription-real-time fluorescence quantitative PCR. Results Compared with the control group (6.50 ± 2.57) s, the mean latency of the platform (17.90 ± 7.71) and (16.20 ± 4.74) s in the medium and high dose group was significantly longer on the 5th d navigation test (P <0.05). Microarray scan revealed that AMPA1 mRNA expression was 2.052 times more than that of the control group. Fluorescence quantitative PCR showed that AMPA1 mRNA was up-regulated 4.257-fold. Conclusion Benzo [a] pyrene affects spatial learning and memory in rats and significantly induces the high expression of AMPA1 in hippocampus.