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AIM:To study the in vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice.METHODS:Aqueous decoction and methanol leaf extracts were tested for their ability to reduce croton oil-induced edema in the mouse ear after topical application.The methanol leaf extract dose-dependently inhibited the croton oil-induced ear edema in mice(ID50<500μg·cm 2).A bioassay-guided liquid–liquid fractionation of this methanol extract gave four active fractions:water insoluble(F1),hexane(F2),ethyl acetate(F3)and water(F4).RESULTS:The hexane fraction showed a very high activity(42.1%inhibition at0.7μg·cm 2)as compared to the control.The other fractions were less active(F1:56.1%at 506.2μg·cm-2;F3:57.3%at 289.3μg·cm 2;and F4:31.9%for 203.8μg·cm 2)while indomethacin gave 48.8%of inhibition at 90μg·cm 2.The activity of F1 and F3 may be at least in part explained by the presence of anti-inflammatory flavonoids,while the activity was not correlated to the tannin contents.No compounds were detected in the most active F2 fraction.CONCLUSIONS:The results give a rational support to the traditional use of T.divaricata in tropical India as anti-inflammatory agent.
AIM: To study the in vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice. METHODS: Aqueous decoction and methanol leaf extracts were tested for their ability to reduce croton oil-induced edema in the mouse ear after topical application. methanol leaf extract dose-dependently inhibited the croton oil-induced ear edema in mice (ID50 <500 μg · cm 2). A bioassay-guided liquid-liquid fractionation of this methanol extract gave four active fractions: water insoluble (F1) F2), ethyl acetate (F3) and water (F4) .RESULTS: The hexane fraction showed a very high activity (42.1% inhibition at 0.7 μg · cm 2) as compared to the control. 56.1% at 506.2 μg · cm -2; F3: 57.3% at 289.3 μg · cm 2; and F4: 31.9% at 203.8 μg · cm 2) while indomethacin gave 48.8% inhibition at 90 μg · cm 2. The activity of F1 and F3 may be at least in part explained by the presence of anti-inflammatory flavonoids, while the activity was not correlated to the tann in contents. No compounds were detected in the most active F2 fractions. CONCLUSIONS: The results give a rational support to the traditional use of T.divaricata in tropical India as anti-inflammatory agent.