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经生物学方法鉴定，小麦－中间偃麦草异附加系Ｚ１高抗我国特有的大麦黄矮病毒ＧＰＶ株系，用１０４个随机引物对Ｚ１及其亲本中７９０２的基因组进行ＲＡＰＤ分析，发现其中的引物ＯＰＳ－１６能从Ｚ１中扩增１个约１．７Ｋｂ的特异性片段。用引物ＯＰＳ－１６扩增Ｚ１、中７９０２、中间偃麦草、中５，同样也从Ｚ１、中间偃麦草、中５中能稳定地扩增此１．７Ｋｂ片段。对Ｚ１的抗、感病单株的自交后代进行ＲＡＰＤ分析，发现在所测的抗病株的自交后代中能稳定地扩增此１．７Ｋｂ片段，而在感病株的自交后代中则不能扩增。
The results showed that wheat-Thinopyrum intermedium Z1 was highly resistant to the barley yellow dwarf virus GPV strain in our country. RAPD analysis of the genome of 7902 in Z1 and its parents was carried out with 104 random primers. The results showed that the primers OPS-16 can amplify a specific fragment of about 1.7 Kb from Z1. The 1.7kb fragment was amplified from Z1, middle 7902, middle thinopyrum, middle 5 with the primer OPS-16 and also from Z1, The RAPD analysis of the selfing offspring of the resistant and susceptible Z1 plants showed that the 1.7Kb fragment could be stably amplified in the selfing offspring of the tested resistant plants and in the selfing offspring of susceptible plants In the can not be amplified.