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目的:了解慢性活动性丙型肝炎肝组织中病毒的复制水平和血液与肝组织中 HCV RNA 含量的相关性,并探索分枝 DNA(branched DNA,bDNA)探针法对肝组织 HCV RNA 定量的可行性。方法:用 bDNA 探针法对38例慢性活动性丙型肝炎病人的血清及肝组织 HCV RNA 进行定量分析,并与竞争性逆转录聚合酶链反应(CRT-PCR)方法进行比较。结果:21份血清 HCV RNA 均高于 cut off 值;所有病例肝组织中均有病毒存在;血清中的病毒含量与肝组织中的病毒含量之间存在阳性相关;两种方法检测结果存在阳性相关。结论:bDNA 探针法对肝组织 HCV RNA 定量具有可行性;血清中病毒含量在一定意义上可反映肝组织中病毒的复制水平;bDNA 探针法与 CRT-PCR 法具有良好的相关性。
OBJECTIVE: To investigate the relationship between viral replication and hepatic HCV RNA in patients with chronic active hepatitis C and to explore the effect of branched DNA (bDNA) probe on the quantification of hepatic HCV RNA feasibility. Methods: The serum and liver HCV RNA of 38 chronic active hepatitis C patients were quantitatively analyzed by bDNA probe method and compared with competitive reverse transcription polymerase chain reaction (CRT-PCR). Results: The serum HCV RNA in all 21 cases were higher than the cut off value. In all cases, there was virus in the liver tissue. There was a positive correlation between the virus content in the serum and the virus content in the liver tissue. There was a positive correlation between the two methods . Conclusion: The bDNA probe method is feasible to quantify HCV RNA in liver tissue. The virus content in serum can reflect the level of virus replication in liver tissue in a certain sense. The bDNA probe method has a good correlation with CRT-PCR method.