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目的:建立同步荧光扫描导数法测定延胡索药材中原阿片碱含量的方法。方法:采用同步荧光扫描导数法以Δλ=10 nm,激发波长扫描范围200～400 nm,扫描结果经一阶导数处理,在290 nm峰位波长处采用标准加入法测定原阿片碱的荧光强度。结果:原阿片碱在1.6～12.00μg.mL-1浓度范围之间线性关系良好,r=0.9991。原阿片碱加样回收率为95.2%,RSD为1.1%。结论:该方法快速准确,灵敏度高,选择性好,可用于延胡索药材中原阿片碱的含量测定。
OBJECTIVE: To establish a synchronous fluorescence scanning derivative method for the determination of protopine in Cynanum chrysogenum. Methods: The fluorescence intensity of protoporphyrin was determined by standard addition method with synchronous fluorescence scanning derivative method at Δλ = 10 nm and excitation wavelength range of 200-400 nm. The first derivative was used for the scan. Results: Protopine showed a good linearity in the concentration range of 1.6 ~ 12.00μg.mL-1, r = 0.9991. Protoporphyrinogen sample recovery was 95.2% with RSD of 1.1%. Conclusion: The method is rapid, accurate, sensitive and selective. It can be used to determine the content of protopine in Corydalis yanhusuo.