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目的:探讨一种人工合成的纤维蛋白原降解肽片段RGDS对脓毒性休克大鼠心肌肌浆网钙转运功能的影响。方法:大鼠盲肠结扎穿孔术后4h和14h分两次尾静脉注射RGDS 5μmol·kg~(-1)。制备大鼠心肌肌浆网(SR)膜;测定SR Ca~(2+)摄取和[~3H]ryanodine受体结合功能。结果:RGDS组大鼠心肌SR摄Ca~(2+)率及摄Ca~(2+)量分别较休克组提高104％(P<0.01)和12％(P<0.05),而心肌SR钙释放通道-[~3H]ryanodine受体结合B_(max)和K_d值没有明显变化。同时RGDS还可以减轻休克大鼠心肌组织钙聚积。结论:RGDS提高休克大鼠心肌SR Ca~(2+)摄取功能,维持心肌细胞钙稳态,具有心肌保护作用。
AIM: To investigate the effect of a synthetic fibrinogen peptide fragment RGDS on calcium transport in sarcoplasmic reticulum of septic shock rats. Methods: RGDS 5 μmol · kg -1 was injected into the caudal vein 4 h and 14 h after cecal ligation and puncture in rats. The sarcoplasmic reticulum (SR) membrane of rat myocardium was prepared. The uptake of SR Ca 2+ and the binding function of [~ 3H] ryanodine receptor were measured. Results: Compared with the shock group, the Ca 2+ content and the Ca 2+ uptake in the SR group increased by 104% (P <0.01) and 12% (P <0.05), respectively, in SRDS group, while SR Release channel - [~ 3H] ryanodine receptor binding B_ (max) and K_d values did not change significantly. At the same time RGDS can also reduce the myocardial accumulation of calcium in shock rats. Conclusion: RGDS can improve SR Ca 2+ uptake in myocardium and maintain the calcium homeostasis of cardiomyocytes in rats with cardioprotection.