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目的建立气相色谱法同时测定雌性柞蚕蛾中棕榈酸、棕榈油酸、硬脂酸、油酸、亚油酸、α-亚麻酸6种成分的含量。方法样品甲酯化后,采用Agilent VF-23 ms毛细管柱(30 m×0.25 mm,0.25μm),柱温程序升温,进样口:250℃,FID检测器:300℃,流速:0.6 m L·min~(-1),进样量:1μL。结果棕榈酸、棕榈油酸、硬脂酸、油酸、亚油酸、α-亚麻酸质量浓度分别在0.032~0.288、0.0064~0.058、0.0064~0.058、0.049~0.443、0.015~0.137、0.083~0.745 g·L~(-1)内呈良好的线性关系(r≥0.999 1),平均回收率为97.8%~99.6%(RSD≤1.7%,n=9)。结论该方法可用于控制雌性柞蚕蛾药材和饮片的质量。
Objective To establish a gas chromatographic method for simultaneous determination of six components of palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid and α-linolenic acid in female tussah moths. Methods After the sample was methyl esterified, the column was programmed with an Agilent VF-23 ms capillary column (30 m × 0.25 mm, 0.25 μm). The temperature of the column was raised. The inlet temperature was 250 ℃, the FID detector was 300 ℃, the flow rate was 0.6 m L · Min ~ (-1), injection volume: 1μL. Results The concentrations of palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid and α-linolenic acid were in the range of 0.032-0.288, 0.0064-0.0580, 0.0064-0.0580, 0.049-0.443, 0.015-0.137 and 0.083-0.745 respectively The average recovery was 97.8% ~ 99.6% (RSD≤1.7%, n = 9). Conclusion This method can be used to control the quality of female tussah moths medicinal herbs and Pieces.