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目的探讨类风湿性关节炎(RA)患者关节腔积液中淋巴毒素β受体(LTβR)信号通路相关分子的表达情况,了解LTβR信号通路与类风湿性关节炎之间的关联性。方法利用实时荧光定量PCR技术检测类风湿性关节炎患者(22例)和骨关节炎患者(25例)关节腔积液标本中LTβR mRNA、LTαmRNA、LTβmRNA、p65 mRNA、Rel B mRNA的表达情况,分析LTβR信号通路相关分子在RA和骨关节炎患者中表达差异。结果类风湿性关节炎组患者关节腔积液中LTβR mRNA和Rel B mRNA的表达高于骨关节炎组,差异均有统计学意义(Z值分别为-4.399、-2.167,P<0.05),而LTαmRNA、LTβmRNA及p65 mRNA相对含量在2组间差异无统计学意义(Z值分别为-1.595、-1.461、-1.493,P>0.05)。结论 LTβR mRNA、Rel B mRNA在类风湿性关节炎患者关节腔积液中高表达,在类风湿性关节炎患者诊断中具有一定的价值,提示其可能在类风湿性关节炎的发生、发展过程中发挥一定的作用,且LTβR信号通路可能以与配体结合后激活非经典NF-κB途径的方式参与作用。
Objective To investigate the expression of lymphatic toxin beta receptor (LTβR) signaling pathway in patients with rheumatoid arthritis (RA) and to find out the correlation between LTβR signaling pathway and rheumatoid arthritis. Methods The expression of LTβR mRNA, LTαmRNA, LTβmRNA, p65 mRNA and Rel B mRNA in the synovial fluid samples of rheumatoid arthritis (22 cases) and osteoarthritis patients (25 cases) were detected by real-time fluorescence quantitative PCR. To analyze the expression of LTβR signaling pathway related molecules in patients with RA and osteoarthritis. Results The expressions of LTβR mRNA and Rel B mRNA in the synovial fluid of patients with rheumatoid arthritis were significantly higher than those of patients with osteoarthritis (Z = -4.399, -2.167, P <0.05, respectively) There was no significant difference in the relative contents of LTα mRNA, LTβ mRNA and p65 mRNA between the two groups (Z values were -1.595, -1.461, -1.493, P> 0.05). Conclusion The expression of LTβR mRNA and Rel B mRNA in patients with rheumatoid arthritis is highly correlated with the diagnosis of rheumatoid arthritis, suggesting that they may play an important role in the pathogenesis of rheumatoid arthritis Play a certain role, and the LTβR signaling pathway may be involved in ligand activation and activation of non-classical NF-κB pathway.