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目的 探讨己酮可可碱 (pentoxifyline ,PTX)对NOD(non obesediabetic)小鼠 1型糖尿病的影响及其机制。方法 采用动物模型NOD鼠 ,PTX处理后检测血糖、尿糖及糖尿病发病率 ,HE染色观察胰岛炎 ,并用RT PCR法检测胰腺干扰素γ(IFN γ)、肿瘤坏死因子α(TNF α)、白介素 10 (IL 10 )mRNA的表达。结果 PTX组糖尿病发生率 (30 .0 % )低于对照组 (6 7.9% ) ,P <0 .0 1;胰岛炎程度也减轻P <0 .0 0 1;胰腺IFN γ、TNF αmRNA的表达较对照组降低 ,P <0 .0 5 ;3项变化均具显著性。而IL 10mRNA的表达则无显著改变。结论 PTX可预防NOD小鼠发生糖尿病 ,其机制可能与纠正Th1与Th2型细胞因子比例失衡有关。
Objective To investigate the effect of pentoxifylline (PTX) on type 1 diabetes mellitus (NOD) and its mechanism. Methods Animal models of NOD mice were used to detect the incidence of blood glucose, urine sugar and diabetes mellitus after treatment with PTX. Insulitis was observed by HE staining. The levels of IFN-γ, TNF α, interleukin-1 10 (IL 10) mRNA expression. Results The incidence of diabetes mellitus (30.0%) in PTX group was lower than that in control group (6.9%), P <0.01; the degree of insulitis was also decreased P <0.01. The expression of IFN-γ and TNF-α mRNA in pancreas Lower than the control group, P <0. 05; 3 changes were significant. However, there was no significant change in IL 10 mRNA expression. Conclusions PTX can prevent the development of diabetes in NOD mice. The mechanism may be related to the imbalance of Th1 and Th2 cytokines.