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目的:筛选测定蔓荆子种子生活力的最佳方法,明确生活力与发芽率之间的相关性。方法:以5个不同产地的蔓荆子作为实验材料,分别用四氮唑染色法、红墨水染色法、BTB指示剂法测定种子的生活力。测定不同培养床(滤纸、海绵、沙子、蛭石)和不同温度(15,20,25,30,30/20,25/15℃)对发芽率的影响,优化种子发芽条件。结果:四氮唑染色法测定种子的生活力是3种测定方法中最准确的,在蛭石培养床、30℃恒温培养条件下,蔓荆子的发芽率最高。四氮唑染色法测定的蔓荆子生活力与发芽力比值为0.992,存在显著的正相关性,进一步求得蔓荆子四氮唑测定生活力X与发芽率Y的回归方程为Y=0.967X+6.038。结论:四氮唑染色法是蔓荆子种子生活力测定的最佳方法,生活力与发芽率呈显著的正相关。
OBJECTIVE: To screen the best method to determine the viability of Viticis indica seeds, and clarify the correlation between viability and germination rate. Methods: Vitex as a test material in five different producing areas, respectively, by tetrazolium staining, red ink staining, BTB indicator method of measuring the viability of the seed. The effects of different culture beds (filter paper, sponge, sand and vermiculite) and different temperature (15,20,25,30,30 / 20,25 / 15 ℃) on the germination rate were determined, and the seed germination conditions were optimized. Results: The viability of the seed was determined by the method of tetrazolium staining. Among the three methods, the germination rate of the seed was the highest in the culture medium of vermiculite at 30 ℃. The ratio of viability and germination percentage of Vitex negundo determined by tetrazolium method was 0.992, and there was a significant positive correlation between them. Further, the regression equation for determining the viability X and the germination rate Y of Vitexin tetrazolium was Y = 0.967 X + 6.038. Conclusion: Tetrazole staining is the best method to determine the seed viability of Viticis. There is a significant positive correlation between viability and germination rate.