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目的明确雷丸菌丝生长的影响因素,为人工栽培提供依据。方法在不同温度、碳源、氮源、p H、培养基质等培养条件下测定雷丸菌丝日生长量。结果 25℃、p H 6.0~7.0条件下菌丝生长快;1%~3%的碳源,菌丝生长速率差异不显著;蛋白胨、酵母粉等氮源对菌丝生长有抑制作用,玉米粉无抑制;竹叶为雷丸栽培种较适宜的栽培主料,玉米粉的促生长作用明显。结论温度、氮源和基质对雷丸菌丝生长的影响较明显。
Objective To clarify the influencing factors of mycelium growth in Leiwan and provide the basis for artificial cultivation. Methods The mycelial growth rate of M. auspiris was determined under different culture conditions, such as temperature, carbon source, nitrogen source, p H and culture medium. Results The mycelial growth was rapid at pH 6.0 ~ 7.0 at 25 ℃. The growth rate of hypha was not significantly different between 1% ~ 3% carbon sources. The nitrogen sources such as peptone and yeast extract could inhibit the growth of mycelium. No inhibition; Bamboo leaves Leiwan cultivars more appropriate materials for cultivation, corn flour, the role of promoting growth significantly. Conclusion The effects of temperature, nitrogen source and substrate on Mycelia growth of Radix Margueris are obvious.