目的探讨新鲜羊膜联合自体角膜缘干细胞、唇黏膜移植治疗重度睑球粘连的临床疗效。方法对15例15眼重度睑球粘连患者进行睑球粘连松解后,应用自体唇黏膜移植进行睑结膜和穹窿部结膜的重建,新鲜羊膜和自体角膜缘干细胞移植重建球结膜和角膜表面。结果随访6～18个月。15例中9例治愈,4例好转,2例无效。结论对于较严重的睑球粘连,新鲜羊膜联合自体角膜缘干细胞、唇黏膜移植是重建眼表的有效方法。 Objective To investigate the clinical efficacy of fresh amniotic membrane combined with limbal stem cells and lip mucosa transplantation in the treatment of severe symblepharon. Methods Fifteen patients (15 eyes) with severe symblepharon were treated with symblepharon release. Autologous lip mucosa transplantation was used to reconstruct conjunctiva of conjunctiva and fornix. Fresh amniotic membrane and autologous limbal stem cell transplantation were used to reconstruct the conjunctiva and corneal surface. The results were followed up for 6 to 18 months. Of the 15 cases, 9 were cured, 4 were improved, and 2 were ineffective. Conclusion For more severe symblepharon, fresh amnion combined with limbal stem cells and lip mucosa transplantation is an effective method to reconstruct ocular surface.