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目的探讨Period2(Per2)基因对细胞生长及放射敏感性的影响。方法体外培养C6神经胶质瘤细胞(C6g liom a ce lls),使用脂质体包裹法将Per2表达质粒(pcDNA3.1-Per2)转导入C6细胞内;以免疫组化及流式细胞术检测Per2表达;通过流式细胞术和克隆形成试验检测稳定转染Per2阳性表达的C6细胞在正常情况下和γ射线照射后的凋亡、生长。结果未经γ射线照射的Per2阳性表达的C6细胞较对照组细胞增殖减慢、凋亡率增加,但γ射线照射后的较对照组细胞增殖速度快、凋亡率减少。结论节律基因Per2过表达会增加肿瘤细胞的凋亡,但在射线照射情况下会降低肿瘤细胞对射线的敏感性。
Objective To investigate the effect of Period2 (Per2) gene on cell growth and radiosensitivity. Methods C6 glioma cells were cultured in vitro. Per2 expression plasmid (pcDNA3.1-Per2) was transfected into C6 cells by lipofectamine. Immunohistochemistry and flow cytometry Per2 expression; PerkinA-positive C6 cells stably transfected with the Per2 gene were detected by flow cytometry and clonogenic assay for apoptosis and growth under normal conditions and after γ-ray irradiation. Results Compared with the control group, the proliferation of Per2-positive C6 cells was slower than that of the control group, and the apoptosis rate was increased. However, the cells proliferated faster and the apoptosis rate decreased after γ-ray irradiation than that of the control group. Conclusion Perval gene overexpression increases tumor cell apoptosis, but decreases the sensitivity of tumor cells to radiation in the presence of radiation.