将38例胸部恶性实体瘤的肿瘤细胞分别与5-Fu等5～6种化疗药物共同培养,于15h后用国际通用的细胞凋亡检测方法──DNA断端末端标记法（Tunel法）检测肿瘤细胞所发生的凋亡率.并以此判断其对抗肿瘤药物的敏感性。结果表明,经过15h的诱导,肿瘤细胞发生凋亡,凋亡细胞形态表现为核呈斑点状或新月状,凋亡率因药物种类不同而异。由于该方法是检测细胞凋亡的早期形态,特别是可在荧光显微镜下选择性地直接观察受药物作用后发生凋亡的肿瘤细胞,故可避免由于组织细胞和血细胞等非肿瘤细胞的污染而造成对判断结果的干扰。这是首次以细胞凋亡为指标作抗肿瘤药敏实验,本法具有简便、快速、准确的特点。 38 cases of malignant tumors of the thoracic tumors were co-cultured with 5-Fu and other 5 to 6 chemotherapeutic drugs. After 15 hours, they were detected by the universal method of apoptosis detection, the method of the DNA stump end (Tunel method). The rate of apoptosis that occurs in tumor cells, and to determine its sensitivity to anti-tumor drugs. The results showed that after 15 h induction, the tumor cells were apoptotic. The appearance of apoptotic cells was spotted in spots or crescents. The apoptotic rate varied with different drug types. Since this method is to detect the early form of apoptosis, in particular, a tumor cell that undergoes apoptosis after selective action of the drug can be selectively observed directly under a fluorescence microscope, so that non-tumor cells such as tissue cells and blood cells can be prevented from being contaminated. Cause interference with the judgment result. This is the first time that apoptosis is used as an indicator for anti-tumor susceptibility testing. This method is simple, rapid, and accurate.