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目的 探讨鉴别胸水性质的酶学指标。方法 采用分光光度法检测胸水中的乳酸脱氢酶 (LDH)、腺苷脱氨酸(ADA) ,采用电泳法检测胸水中的乳酸脱氢酶同工酶。结果 恶性胸水和结核性胸水与炎性胸水之间LDH活性有显著性差异 (P <0 .0 5 )。三者之间ADA活性均有显著性差异 (P <0 .0 5 )。结论 胸水中LDH、LDH同工酶、ADA是临床上鉴别胸水性质有用的指标 ,联合检测可以提高恶性胸水、结核性胸水的阳性率
Objective To explore the enzymatic properties of pleural effusion. Methods Lactate dehydrogenase (LDH) and adenosine deaminase (ADA) in pleural fluid were detected by spectrophotometry. The lactate dehydrogenase isoenzymes in pleural fluid were detected by electrophoresis. Results There was a significant difference in LDH activity between malignant and tuberculous pleural effusion and inflammatory pleural effusion (P <0.05). ADA activity among the three were significantly different (P <0. 05). Conclusions LDH, LDH isoenzymes and ADA in pleural effusion are useful indicators for the differential diagnosis of pleural effusion. Combined detection can improve the positive rate of malignant pleural effusion and tuberculous pleural effusion