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用改进的斑点法检测柑桔黄龙病类细菌、香蕉束顶病毒(BBTV),均获满意结果.阳性反应在硝基纤维转移膜上形成深浅不等的褐色斑点.检测健康柑桔、香蕉粗汁液,获阴性结果,在上述固相载体上不形成有色物质.在缓冲液TBS中加入2％TritonX-100和1％国产蛋白片,作为改进后的封闭缓冲液,封闭硝基纤维转移膜上未结合抗原部位,效果较好.斑点法检测活体香蕉束顶病样品及-30℃冷冻保存6个月香蕉束顶病样品,同样获满意结果.斑点法快速、准确、简单、灵敏度高、特异性强,可用于植物检疫,存在于硝基纤维转移膜上的检疫结果可长期保存.
The improved spot detection of citrus Huanglongbing bacteria, banana bunchy top virus (BBTV), were satisfactory results positive reaction in the nitrocellulose transfer film to form varying shades of brown spots.Studies on healthy citrus, banana coarse Juice, negative results, no formation of color material on the solid support described above TBS was added to the buffer TBS 2% TritonX-100 and 1% of the domestic protein pieces as an improved blocking buffer, blocking the nitrocellulose transfer membrane Unbound antigen site, the effect is better.Flag test banana bunchy top in vivo samples and frozen at -30 ℃ for 6 months banana bunchy top samples, the same satisfactory results.Flat method is fast, accurate, simple, high sensitivity, specificity Strong, can be used for phytosanitary, quarantine results exist in the nitrocellulose transfer membrane can be long-term preservation.