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本试验利用两种有效的诱变剂PYM和EMS,在大麦减数分裂早前期对幼穗进行注射处理,通过对其M_1花粉母细胞染色体畸变率、花粉育性,结实率及M_2性状变异等的研究表明,幼穗处理能显著提高诱变效果,增加染色体畸变率和M_2基因突变率,同时也提高了某些有益性状的变异频率。因此,认为减数分裂早前期是诱变处理的适宜时期。本试验首次采用注射处理的方法,此法能弥补常见浸泡法所造成的人为损伤过大的缺点,提高诱变效果,而且方法简便易行,是一种行之有效的方法。
In this study, two effective mutagens, PYM and EMS, were used to treat the young ear in the early stage of meiosis in barley. The chromosome aberration rate, pollen fertility, seed setting rate and M_2 variation of M_1 pollen mother cell were studied Studies have shown that panicle treatment can significantly improve the mutagenesis effect, increase the chromosome aberration rate and M 2 gene mutation rate, but also improve the frequency of mutation of some beneficial traits. Therefore, it is considered that the early stage of meiosis is the appropriate period for mutagenic treatment. This test for the first time using the injection method, this method can make up for the common man-made damage caused by excessive shortcomings, to improve the mutagenesis effect, and the method is simple and easy, is an effective method.