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显微光子计数成像系统是极其灵敏的弱光探测成像系统，为使其应用进一步深入微观领域，噪声问题是首先要解决的难题。通过噪声来源分析，光路、机械结构的合理设计，使噪声降低到光子计数成像系统的探测灵敏域之下；并在噪声极低的条件下，进行了一种同位素微弱光源的稳定性、均匀性的研究，发现这种微弱光源性能稳定、发光均匀，可以作为定量测量的标准，为以后的微弱发光实验进行定量分析；光子计数成像系统的合理噪声抑制以及定量测量标准的确立，将在微光探测方面产生重要的影响。
Microscopic photon counting imaging system is extremely sensitive weak light detection imaging system, in order to further its application in the micro-field, the noise problem is the first to solve the problem. Through the noise source analysis, the reasonable design of optical path and mechanical structure, the noise is reduced below the detection sensitivity of the photon counting imaging system. Under the conditions of extremely low noise, the stability and uniformity of a weak isotope source The results show that this weak light source has stable performance and uniform luminescence and can be used as a quantitative measurement standard for quantitative analysis of later weak luminescence experiments. The reasonable noise suppression and quantitative measurement standard of photon counting imaging system are established. Detection has a significant impact.