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5. CLONING AND HIGH EXPRESSION OF FULL LENGTH hTRF1 IN E. COLI

CLONING AND HIGH EXPRESSION OF FULL LENGTH hTRF1 IN E. COLI

来源 :中国癌症研究：英文版 | 被引量 : 0次 | 上传用户：mkkkj2009

【摘 要】

：

Objective: To express human telomeric repeat binding factor (TRF1) at high level in E. coli. Method: Two primers were designed with KpnⅠand EcoRⅠ sites respe

【作 者】

：

HUANG He 

【出 处】

：

中国癌症研究：英文版

【发表日期】

：

2001年3期

【关键词】

：

TRF1 克隆 蛋白质 TRF1 Clone Protein 

【基金项目】

：

国家自然科学基金

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Objective: To express human telomeric repeat binding factor (TRF1) at high level in E. coli. Method: Two primers were designed with KpnⅠand EcoRⅠ sites respectively, TRF1 cDNA fragments was amplified and cloned into plasmid pET29a, each step was confirme

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