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目的观察活血抗生滴丸对佐剂型关节炎(AA)大鼠血清、滑膜血管内皮生长因子/内皮抑素(VEGF/en- dostatin)失衡的影响。方法建立大鼠AA模型,将Wistar大鼠随机分为活血抗生滴丸低、中、高(0.63,1.25,2.5 g·kg~(-1))剂量组、环孢霉素A组(10 mg·kg~(-1))及模型组。于造模后第19天开始给药,每日1次,连续灌胃30 d。检测各组大鼠关节肿胀度、关节炎指数积分。ELISA法检测各组大鼠血清VEGF、endostatin蛋白的表达。免疫组化法检测各组大鼠滑膜组织VEGF、endostatin蛋白的表达。计算VEGF/endostatin比值。结果活血抗生滴丸高剂量组和环孢霉素A组可降低大鼠关节肿胀度、关节炎指数积分,降低大鼠血清和滑膜VEGF含量,提高endostatin含量,降低VEGF/endostatin比值,与模型组比较,差异有显著性(P<0.05或P<0.01)。结论活血抗生滴丸可抑制AA大鼠炎症发展,其机制可能与活血抗生滴丸调控VEGF/endostatin的失衡而抑制滑膜组织血管生成相关。
Objective To observe the effect of Huoxue Kangsheng Dripping Pills on the imbalance of serum and synovial vascular endothelial growth factor/endostatin (VEGF/en-dostatin) in rats with adjuvant arthritis (AA). Methods Rat AA model was established. Wistar rats were randomly divided into low, medium, and high doses of Huoxue Kangsheng Pills (0.63, 1.25, 2.5 g·kg -1) and cyclosporine A (10 mg). ·kg~(-1)) and model groups. The administration was started on the 19th day after the model was established, and once a day for 30 days. Rat joint swelling and arthritis index scores were measured in each group. ELISA was used to detect the expression of serum VEGF and endostatin protein in each group. The expression of VEGF and endostatin protein in the synovial tissue of each group was detected by immunohistochemistry. Calculate the VEGF/endostatin ratio. Results Huoxue Kangsheng Dripping Pill high-dose group and cyclosporine A group can reduce joint swelling, arthritis index integral, reduce serum and synovial membrane VEGF levels, increase endostatin content, and reduce VEGF/endostatin ratio in rats. There was a significant difference between the groups (P<0.05 or P<0.01). Conclusion Huoxue Kangsheng Dripping Pills can inhibit the development of inflammation in AA rats. The mechanism may be related to the imbalance between VEGF/endostatin regulated by Huoxue Kangsheng Dripping Pills and the inhibition of angiogenesis in synovial tissue.