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目的探究乙型肝炎病毒两对半检查与HBV-DNA之间的相互关系。方法选取本院2011~2012年收治的肝炎患者125例,采取患者的血清标本,采用酶联免疫法进行乙型肝炎病毒标志物的检测,及“两对半”检测法,同时利用荧光聚合链反应定量检测患者的HBV-DNA。将两者结果进行比较。结果经荧光聚合链定量检测法检测有80例患者HBV-DNA定量为阳性,检出率为64.0%,其中HBsAg、HBeAg、抗-HBc标志物阳性的HBV-DNA的检出率最高,HBsAg、抗-HBc次之,抗-HBc最低。结论 HBV-DNA与病毒的活动性和感染性相关联,确定HBV-DNA可了解乙肝病毒的复制和感染的状况,可作为不同临床类型肝炎患者的辅助检查,可作为判断HBV感染者病毒是否有活动和较大传染性的新标志。
Objective To explore the relationship between hepatitis B virus two-and-a-half examination and HBV-DNA. Methods A total of 125 hepatitis patients admitted to our hospital from 2011 to 2012 were enrolled in this study. Serum samples from patients were collected. Enzyme-linked immunosorbent assay (ELISA) was used to detect hepatitis B virus (HBV) markers and “two and a half” detection methods. Fluorescence Polymerase chain reaction for quantitative detection of HBV-DNA in patients. Compare the two results. Results The detection rate of HBV-DNA in 80 patients was 64.0% by fluorescence quantitative PCR. The positive rate of HBV-DNA was the highest in HBsAg, HBeAg and anti-HBc. HBsAg, Anti-HBc followed, anti-HBc lowest. Conclusion HBV-DNA is associated with the activity and infectivity of the virus. HBV-DNA can be used to understand the replication and infection status of HBV. It can be used as an auxiliary examination for patients with different clinical types of hepatitis and can be used as a marker to determine whether there is a Activities and new signs of greater contagion.