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目的:探讨谷胱甘肽转硫酶(GSTs)P1基因型与湖北汉族人群溃疡性结肠炎(UC)的关系。方法:在270例UC患者和623例正常对照中,先采用聚合酶链反应技术(PCR)检测GSTP1基因多态性;然后随机选取65例UC患者和90例性别、年龄相匹配的健康对照者,采用酶联免疫吸附(ELISA)法检测空腹血浆GSTpi水平。结果:与正常对照组相比,GSTP1(VaL/VaL)基因型频率在UC患者中明显增高(48.89%比34.35%,P=0.0004)。进一步根据UC临床症状分层分析,在远端结肠炎患者中GSTP1(VaL/VaL)基因型的分布频率高于广泛结肠炎(57.64%比29.27%,P=0.001);而GSTP1突变基因型与UC患者病情严重程度无关(29.54%比45.46%,P=0.065)。与UC患者相比较,正常对照组中血浆GSTpi水平明显增高[(42.37±22.47)mmol/L比(65.40±51.59)mmol/L,P=0.009]。结论:突变的GSTP1基因型及血浆GSTpi水平与湖北汉族UC明显相关。
Objective: To investigate the relationship between glutathione S-transferase (GSTs) P1 genotypes and ulcerative colitis (UC) in Hubei Han population. Methods: Polymorphisms of GSTP1 gene were detected by polymerase chain reaction (PCR) in 270 patients with UC and 623 normal controls. Then, 65 patients with UC and 90 healthy controls with the same age were randomly selected , The level of GSTpi in fasting plasma was detected by enzyme-linked immunosorbent assay (ELISA). Results: Compared with the normal control group, the frequency of GSTP1 (VaL / VaL) genotype was significantly higher in patients with UC (48.89% vs 34.35%, P = 0.0004). According to the stratified analysis of clinical symptoms of UC, the distribution frequency of GSTP1 (VaL / VaL) genotype in patients with distal colitis was higher than that of extensive colitis (57.64% vs. 29.27%, P = 0.001). The genotypes of GSTP1 mutation The severity of UC patients had no relationship (29.54% vs 45.46%, P = 0.065). Compared with UC patients, plasma GSTpi level was significantly higher in the control group (42.37 ± 22.47 mmol / L, 65.40 ± 51.59 mmol / L, P = 0.009). Conclusion: The genotypes of GSTP1 and plasma GSTpi are significantly correlated with UC in Hubei Han.