论文部分内容阅读
目的:探讨不同剂量放射线对口腔鳞状细胞癌(OSCC)细胞中核因子-κB(nuclear factor-κB,NF-κB)p65信号转导蛋白表达的影响。方法:采用人舌鳞状细胞癌Tca8113细胞,分为6个剂量组,分别为0y、2、4、6、8、10Gy,在照射后的不同时间点(1,3,6,10,24,48h)应用免疫细胞化学检测NF-κB p65的表达情况,应用Leica Qwin Plus图像分析系统对图像进行分析,测其灰度值,并应用SPSS10.0软件对数据进行X2检验。结果:免疫细胞化学方法显示不同剂量组的平均灰度值与0Gy组相比均为P<0.05,差异均具有统计学意义,而不同时间点组的平均灰度值相互比较,3h组的平均灰度值与其他时间点组相比均为P<0.05,差异有显著性。结论:放射线可以激活NF-κB p65信号转导通路,此信号转导通路在肿瘤的放射治疗中可能具有重要意义。
Objective: To investigate the effect of different doses of radiation on the expression of nuclear factor-κB (NF-κB) p65 signal transducers in oral squamous cell carcinoma (OSCC) cells. Methods: Human tongue squamous cell carcinoma Tca8113 cells were divided into 6 dose groups (0y, 2, 4, 6, 8 and 10 Gy, respectively) at different time points after irradiation , 48h). Immunocytochemistry was used to detect the expression of NF-κB p65. The image was analyzed by Leica Qwin Plus image analysis system. The gray value of NF-κB p65 was detected. The data was analyzed by SPSS10.0 software. Results: The results of immunocytochemistry showed that the average gray value of different dose groups was significantly lower than that of 0Gy group (P <0.05), and the difference was statistically significant, while the average gray value at different time points were compared with each other, The gray value was P <0.05 compared with other time points, the difference was significant. Conclusion: Radiation can activate NF-κB p65 signal transduction pathway, which may play an important role in the radiation therapy of tumors.