论文部分内容阅读
[Objective] To identify the genuine and false FLOS CHRYSANTHEMI INDICI by HPLC, so as to provide basis for the standardization of traditional Chinese medicine market. [Method] By determining the content of buddleoside and chlorogenic acid, the genuine and false FLOS CHRYSANTHEMI INDICI were identified. HPLC conditions were as follows: mobile phase of methanol-water- glacial acetic acid (V∶ V∶ V=26∶31∶1), detection wavelength of 334 nm, flow rate of 1 ml/min, column temperature of 35 ℃ and sampling amount of 10 μl. [Result] Contents of buddleoside and chlorogenic acid in genuine FLOS CHRYSANTHEMI INDICI were 8.365 and 0.228 mg/g respectively, while which were not detected in false FLOS CHRYSANTHEMI INDICI. [Conclusion] The method established in this study was convenient and feasible, which could be employed to distinguish genuine and false FLOS CHRYSANTHEMI INDICI.
[Objective] To identify the genuine and false FLOS CHRYSANTHEMI INDICI by HPLC, so as to provide basis for the standardization of traditional Chinese medicine market. [Method] By determining the content of buddleoside and chlorogenic acid, the genuine and false FLOS CHRYSANTHEMI INDICI were The HPLC conditions were as follows: mobile phase of methanol-water-glacial acetic acid (V:V:V:26:31:1), detection wavelength of 334 nm, flow rate of 1 ml / min, column temperature of 35 ℃ and the sampling amount of 10 μl. [Result] Contents of buddleoside and chlorogenic acid in genuine FLOS CHRYSANTHEMI INDICI were 8.365 and 0.228 mg / g respectively, while which were not detected in false FLOS CHRYSANTHEMI INDICI. [Conclusion] The method established in this study was convenient and feasible, which could be employed to distinguish genuine and false FLOS CHRYSANTHEMI INDICI.