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目的 探讨内皮素 ( ET)和一氧化氮 ( NO)在妊娠高血压综合征 ( PIH)患者的新生儿脐血的变化。方法 使用放免法测定 ET;采用硝酸根还原酶法结合 Griess试剂测定 NO的稳定代谢产物亚硝酸基 ( NO- 2 )和硝酸基 ( NO- 3)的含量。对 2 1例 PIH患者的新生儿和 2 4例健康孕妇的正常新生儿脐血进行了测定。结果 中、重度 PIH患者新生儿脐血 ET显著高于对照组 ,而 NO- 2 和 NO- 3含量显著低于对照组 ( P<0 .0 5 )。中、重度 PIH并发胎儿宫内发育迟缓 ( IUGR)者脐血 ET显著高于未并发 IUGR组 ( P<0 .0 1) ,而NO- 2 和 NO- 3显著低于未并发 IUGR者 ( P<0 .0 5 )。结论 ET升高 ,NO降低可能是 IUGR发生的重要环节。
Objective To investigate the changes of neonatal cord blood in patients with pregnancy induced hypertension (PIH) treated with endothelin (ET) and nitric oxide (NO). Methods ET was determined by radioimmunoassay. The contents of nitrite (NO-2) and nitric oxide (NO-3) were determined by nitrate reductase and Griess reagent. Umbilical cord blood was measured in 21 neonates with PIH and 24 normal pregnant women. Results In newborns with severe PIH, cord blood ET was significantly higher than that of the control group, while NO-2 and NO-3 levels were significantly lower than those of the control group (P <0.05). In moderate and severe PIH complicated with intrauterine growth retardation (IUGR), umbilical blood ET was significantly higher than those in uncomplicated IUGR group (P <0.01), while NO- 2 and NO- 3 were significantly lower than those in uncomplicated IUGR (P <0 .0 5). Conclusions ET increased, NO decreased may be an important link in the occurrence of IUGR.