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作者从ｐＳＰ７３中分离出２２１ｈｐ多克隆区整合至ＰＭＷ１１９。从ｐＳＫ６和ｐＳＫ１６中分离出葡糖基转移酶基因ｇｔｆＢ和ｇｔｆＣ．并整合至这一重组质粒中，分别形成ｐＴＦ４１和ｐＴＦ４２。从ｐＶＡ８３８中分离出链球菌复制子并整合至ｐＴＦ４１和ｐＴＦ４２的适当部位．分别构成重组质粒ｐＺＢ１和ｐＺＢ２。ｐＺＢ１和ｐＺＢ２为分别含ｇｔｆＢ或ｇｔｆＣ基因的链球菌－大肠杆菌穿梭质粒。
The authors isolated the 221 hp polyclonal region from pSP73 and integrated it into PMW119. Glucosyltransferase genes gtfB and gtfC were isolated from pSK6 and pSK16. And integrated into this recombinant plasmid to form pTF41 and pTF42, respectively. Streptococcal replicons were isolated from pVA838 and integrated into the appropriate sites of pTF41 and pTF42. Respectively constitute the recombinant plasmids pZB1 and pZB2. pZB1 and pZB2 are Streptococcus-E. coli shuttle plasmids containing gtfB or gtfC genes, respectively.