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目的:建立测定异丙酚血药浓度的方法。方法:应用反相高效液相色谱技术,血浆样品经10％三氯乙酸甲醇溶液沉淀后,离心取上清液,以甲醇-水(85:15)为流动相,流速1mL·min~(-1),进样量50μL,经C_(18)柱分离后,荧光检测,λex=276nm,λem=310nm。结果:在0.1～8.0μg·mL~(-1)浓度范围内,异丙酚峰面积与浓度呈良好的线性关系(r=0.9994)。异丙酚的平均加样回收率大于90％,日内和日间RSD均小于4％。本实验最低检测浓度为0.05μg·mL~(-1),绝对回收率大于70％。结论:本法快速、简便、准确、灵敏,可用于异丙酚的血药浓度监测。
Objective: To establish a method for the determination of propofol plasma concentration. Methods: The RP - HPLC method was used. The plasma samples were precipitated with 10% trichloroacetic acid in methanol and the supernatant was centrifuged. The mobile phase was methanol - water (85:15) and the flow rate was 1 mL · min ~ 1), injection volume 50μL, after C_ (18) column separation, fluorescence detection, λex = 276nm, λem = 310nm. Results: There was a good linear relationship between the peak area of propofol and the concentration in the range of 0.1-8.0 μg · mL -1 (r = 0.9994). The average recovery of propofol was greater than 90%, with daily and intraday RSDs less than 4%. The lowest concentration of this experiment was 0.05μg · mL -1, the absolute recovery was more than 70%. Conclusion: The method is rapid, simple, accurate and sensitive and can be used to monitor plasma concentration of propofol.