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应用新一代高通量测序技术,对紫薇金叶突变体的叶片进行转录组测序和生物信息学分析。本研究中共组装获得45 308条unigenes,平均长度987.51 bp。21 339(47.10%)条可以匹配到蛋白数据库获得注释信息。Unigenes在各数据库中注释的基因数分别为:在COG数据库中有11 512(25.41%)条,在GO数据库中有12 196条(26.92%),在KEGG数据库中有5 709条(27.73%)。通过与NCBI和Uniprot蛋白数据库的比对,共22条叶绿素代谢相关基因和17条类胡萝卜素代谢相关基因被鉴定出来,为突变体基因的筛选提供了候选基因。
A new generation of high-throughput sequencing technology was used to analyze the transcriptome and bioinformatics analysis of leaves of Lagerstroemia speciosa. In this study, a total of 45 308 unigenes were assembled and the average length was 987.51 bp. 21 339 (47.10%) matches to the protein database for comment information. The number of genes annotated by Unigenes in each database was 11 512 (25.41%) in the COG database, 12 196 (26.92%) in the GO database and 5 709 (27.73%) in the KEGG database . Through the comparison with NCBI and Uniprot protein database, a total of 22 chlorophyll metabolism-related genes and 17 carotenoid metabolism-related genes were identified, which provided candidate genes for the screening of mutant genes.