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利用原代培养大鼠肝细胞的方法,研究右旋儿茶素(d-CTC)对四氯化碳(CCl_4)或半乳糖胺(d-GalN)引起肝细胞毒性的作用,并观察该药对肝酶的直接作用。结果表明:d-CTC 0.6～5.0mg/ml可使含10mmol/L CCl_4或5mmol/Ld-GalN肝细胞培养液中的GOT.GPT及LDH活性显著降低,并呈量效关系。d-CTC 2.5～5.0mg/ml可直接抑制LDH及GPT活性,但对GOT无明显影响。其抑酶作用与抗肝毒而降低酶活性比较,有非常显著的差别,提示d-CTC具有较强的抗肝毒作用。
The effect of d-CTC on hepatotoxicity induced by carbon tetrachloride (CCl 4) or galactosamine (d-GalN) was studied by primary culture of rat hepatocytes. Direct effect on liver enzymes. The results showed that the GOT.GPT and LDH activities in 10 mmol / L CCl 4 or 5 mmol / Ld-GalN hepatocytes culture medium with d-CTC at 0.6-5.0 mg / ml were significantly decreased and showed a dose-response relationship. d-CTC 2.5 ~ 5.0mg / ml can directly inhibit LDH and GPT activity, but no significant effect on GOT. The inhibition of its role with anti-hepatotoxicity and reduce enzyme activity, there is a very significant difference, suggesting that d-CTC has a strong anti-hepatotoxic effect.