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应用ＰＣＲ方法，从杂交瘤细胞中扩增鼠抗人膀胱癌抗体重、轻链可变区基因，经ＤＮＡ接头连接后与噬菌粒ｐＣＡＮＴＡＢ５重组，转化大肠杆菌后制备噬菌体抗体文库，通过亲和筛选、再转染、克隆化及鉴定，得到高亲和力抗人膀胱癌噬菌体单链抗体。
The PCR method was used to amplify the antibody heavy and light chain variable region genes of mouse anti-human bladder cancer from hybridoma cells. After being ligated with DNA linker, the recombinant plasmid was recombined with the phagemid pCANTAB5. The phage antibody library was prepared after transformation into E.coli. Screening, then transfected, cloned and identified to obtain high affinity anti-human bladder cancer phage scFv.