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目的:观察钙敏感受体(Ca SR)在大鼠糖尿病性心肌病中的作用。方法:30只雄性Wistar大鼠随机分为对照组、糖尿病4周组和糖尿病8周组(n=10)。高糖高脂饮食4周后腹腔注射STZ(30 mg/kg)建立糖尿病模型。通过透射电镜观察心肌超微结构的变化,通过Western blot检测心肌组织Ca SR、肌浆网Ca2+-ATPase(SERCA)和受磷蛋白(PLN)的蛋白表达。结果:与对照组相比,随着病程的延长糖尿病组大鼠心肌Ca SR、SERCA蛋白表达降低,PLN表达增加,心肌结构损伤逐渐加重。结论:在大鼠糖尿病性心肌病进展过程中Ca SR的表达逐渐降低,并可能导致钙稳态紊乱参与糖尿病心肌病的发生。
Objective: To observe the role of calcium-sensitive receptor (Ca SR) in diabetic cardiomyopathy in rats. Methods: Thirty male Wistar rats were randomly divided into control group, diabetic 4 weeks group and diabetic 8 weeks group (n = 10). High glucose and high fat diet 4 weeks after intraperitoneal injection of STZ (30 mg / kg) to establish a diabetic model. The changes of myocardial ultrastructure were observed by transmission electron microscope. The protein expressions of Ca SR, sarcoplasmic reticulum Ca2 + -ATPase (SERCA) and phospholamban (PLN) in myocardium were detected by Western blot. Results: Compared with the control group, the expression of Ca SR and SERCA protein in diabetic rats decreased, the expression of PLN increased, and the myocardial injury gradually increased with the prolongation of duration. CONCLUSIONS: The expression of Ca SR gradually decreases during the progression of diabetic cardiomyopathy in rats and may lead to the disturbance of calcium homeostasis in the pathogenesis of diabetic cardiomyopathy.