In the adenine-induced renal failure rats,reversibility of renal failure and recovery of bone mineral density (BMD) bydiscontinuation of adenine-rich diet were reported.We think that the effect to bone metabolism with medication may be ableto be evaluated as reinforcement of the BMD recovery.We have so far investigated the Chinese herbal medicine based onHachimi-jio-gan (HJG) which are more effective than HJG alone.In this study,we investigated the effects of our Chineseherbal prescription on BMD in the adenine-treated rats compared to that of vitamin D_3treatment.Young male rats wereadministrated 100 mg/ml adenine for 8 weeks,and they showed renal failure and bone loss.The adenine-treated rats weredivided into the following 3 groups,that is,the group experienced no treatment (conlrol),the group given our Chinese herbalmedicine (HAO),and the group given vitamin D_3 (VD3) medication.It is likely that VD3 medication was less effective forincrease of the femoral BMD than increase of the spinal BMD.In contrast,HAO was effective for increase of the femoralBMD.The VD3 group showed low deoxypyridinoline (Dpd:bone resorption maker) as compared to the control group.However,the HAO group showed same or slightly high Dpd.It is suggested that VD3 may increase BMD by reduction ofbone resorption,while HAO may show effect on BMD by activating bone metabolism.It is indicated that HAO maybecome a curative medicine for bone loss because of the different target site from vitamin D_3. In the adenine-induced renal failure rats, reversibility of renal failure and recovery of bone mineral density (BMD) bydiscontinuation of adenine-rich diet were reported.We think that the effect to bone metabolism with medication may be ableto be evaluated as as BMD recovery.We have so far investigated the Chinese herbal medicine based onHachimi-jio-gan (HJG) which are more effective than HJG alone.In this study,we investigated the effects of our Chineseherbal prescription on BMD in the adenine- treatment symptom. To that of vitamin D_3treatment.Young male rats wereadministrated 100 mg/ml adenine for 8 weeks,and they showed renal failure and bone loss.The adenine-treated rats weredivided into the following 3 groups,that is, the group experienced no treatment (conlrol ), the group given our Chinese herbalmedicine (HAO), and the group given vitamin D_ 3 (VD3) medication.It is likely that VD3 medication was less effective forincrease of the femoral BMD than increase of the s Pinal BMD.In contrast, HAO was effective for increase of the femoralBMD. The VD3 group showed low deoxypyridinoline (Dpd:bone resorption maker) as compared to the control group.However, the HAO group showed same or slightly high Dpd.It is suggested That VD3 may increase BMD by reduction ofbone resorption, while HAO may show effect on BMD by activating bone metabolism.It is indicated that HAO maybecome a curative medicine for bone loss because of the different target site from vitamin D_3.