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目的探讨煤工尘肺支气管肺泡肺灌洗液(BALF)蛋白质含量、细胞成分。方法对煤工尘肺BALF中总蛋白及超氧化物歧化酶(SOD)进行分析,然后对BALF进行聚丙烯酰胺凝胶电泳,对BALF的蛋白质进行分离。对离心后的细胞,应用瑞氏染色法方法进行观察,并应用电镜技术对巨噬细胞进行观察。结果BALF中SOD活力之间的差异有显著性(P<0.05),不同期别BALF中总蛋白含量升高。在此蛋白含量下,进行聚丙烯酰胺凝胶电泳。从图谱上可以辨认出10～20稳定的条带。BALF中炎性细胞总数有明显增多,白细胞总数及其分类中性粒细胞、巨噬细胞和淋巴细胞均有不同程度的升高。在电镜下,巨噬细胞膜较完整,细胞核边缘模糊,偶可见固缩现象,核仁明显。结论煤工尘肺BALF中SOD的活力发生变化,总蛋白含量显著升高,对洗液细胞进行分类,在电镜下观察的巨噬细胞形态可以反映出巨噬细胞在尘肺发病过程中的变化。
Objective To investigate the protein content and cellular components of bronchoalveolar lavage fluid (BALF) in coal workers’ pneumoconiosis. Methods The total protein and superoxide dismutase (SOD) in BALF of coal worker’s pneumoconiosis were analyzed. Then, the BALF protein was separated by polyacrylamide gel electrophoresis. After centrifugation, the Wright staining method was used to observe the macrophages. Electron microscopy was used to observe the macrophages. Results The difference of SOD activity in BALF was significant (P <0.05), and the total protein in BALF was increased in different stages. Under this protein content, polyacrylamide gel electrophoresis was performed. From the map can be identified from 10 to 20 stable band. The total number of inflammatory cells in BALF was significantly increased, the total number of leukocytes and their classification of neutrophils, macrophages and lymphocytes were increased to varying degrees. Under electron microscope, the membrane of macrophages is more complete, the edge of the nucleus is fuzzy, and the phenomenon of shrinkage can be observed even with obvious nucleoli. Conclusion The activity of SOD in BALF of coal worker ’s pneumoconiosis changed and the total protein content increased significantly. The classification of lotion cells showed that the morphology of macrophages observed under electron microscope reflected the changes of macrophages in the pathogenesis of pneumoconiosis.