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实验应用雄性SD大鼠，皮下埋植内置10mg雌二醇（E2）硅胶管3个月致垂体催乳素（prolactin，PRL）瘤后，取PRL瘤细胞进行体外原代培养，并应用原位杂交方法，研究不同剂量的胃泌素释放肽（gastrin－releasingpeptide，GRP）和血管活性肠肽（vasoactiveintestinalpolypeptide，VIP）以及E2对离体培养的垂体PRL瘤细胞PRL基因转录的影响。结果如下：GRP，VIP分别与PRL瘤细胞孵育24h后，10－8mol／L，10－7mol／L的GRP均不影响PRL瘤细胞内PRLmRNA水平，但10-6mol／L的GRP可使胞内PRLmRNA水平下降20％（P＜0．05）。在本实验所采用的浓度范围内，VIP均可升高PRL瘤细胞内PRLmRNA水平，10－8mol／L，10－7mol／L，10－6mol／L的VIP分别使胞内PRLmRNA升高为对照组的1．60，2．10，2．21倍（P＜0．05）。三种浓度的E2分别与PRL瘤细胞孵育48h后，10－8mol／LE2不影响胞内PRLmRNA水平，但10－7mol／L，10－6mol／L的E2则分别可使胞内PRLmRNA升高为对照组的2．80，2．92倍（P＜0．05）。上述结果表明：GRP和VIP可能分别抑制和增强由E2诱致的垂体PRL瘤细胞PRL基因的转录；一定浓度的E2可能直接涉及E2诱发垂体PRL瘤时伴高PRL血症。
Experimental male Sprague-Dawley rats were implanted subcutaneously with 10mg estradiol (E2) silicone tube for 3 months to induce prolactin (PRL) tumor. PRL tumor cells were obtained and cultured in vitro. In situ hybridization METHODS: The effects of different doses of gastrin-releasing peptide (GRP) and vasoactive intestinal polypeptide (VIP) and E2 on PRL gene transcription in cultured pituitary PRL tumor cells were studied. The results were as follows: GRP, VIP and PRL tumor cells incubated 24h, 10-8mol / L, 10-7mol / L of GRP did not affect PRL tumor PRL mRNA levels, but 10-6mol / L of GRP intracellular PRL mRNA levels decreased by 20% (P <0.05). In the concentration range used in this experiment, VIP can increase PRL tumor PRL mRNA levels, 10-8mol / L, 10-7mol / L, 10-6mol / L of VIP were intracellular PRLmRNA increased to control 1.60,2.10,2.21 times (P <0.05). After three concentrations of E2 and PRL tumor cells incubated for 48h, 10-8mol / LE2 did not affect intracellular PRL mRNA levels, but 10-7mol / L, 10-6mol / L of E2 were intracellular PRLmRNA increased Control group 2.80,2.92 times (P <0.05). The above results indicate that GRP and VIP may inhibit and enhance the transcription of PRL gene in pituitary PRL cells induced by E2, respectively. A certain concentration of E2 may be directly involved in E2-induced pituitary PRL tumor with high PRL.