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在日本冲绳,菠萝是一种主要的热带果品作物。这种作物具有高度的自交不亲和性。无刺卡因种在当地有约160年的栽培史,从这一品种中分离自发突变体在育种方面起过重要作用。用组织培养技术进行菠萝育种,首先,可以用杂种第一代的花粉诱导出许多第二代植株,从这些植株容易获得纯系,并会从中发现重组体植株;其次,可以获得许多突变体。组织培养的全过程会增加获得突变体的机会,因为从愈伤组织的单细胞或寡细胞会分化植株,一次只在一个细胞发生突变。此外,在愈伤组织和分化植株中的染色体数目和结构起了变化。同完整植株相比,对培养基或培养组织进行化学诱变剂或辐射;处理较容易。组织培养
In Okinawa, Japan, pineapple is a major tropical fruit crop. This crop has a high degree of self-incompatibility. Since there is about 600 years of cultivation history in the area without the card snail, the isolation of spontaneous mutants from this variety has played an important role in breeding. First, the second generation of plants can be induced from the pollen of the first generation of hybrids. Pure lines can be easily obtained from these plants and the recombinant plants can be found therefrom. Second, many mutants can be obtained. The whole process of tissue culture increases the chances of getting mutants because single or oligo cells from the calluses will differentiate into plants that mutate in only one cell at a time. In addition, the number and structure of chromosomes in callus and differentiated plants have changed. Compared to intact plants, chemical mutagens or radiation are applied to the medium or to the culture tissue; the treatment is easier. Tissue culture