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目的 :观察口腔鳞癌组织中Survivin的表达及其与微血管密度 (microvesseldensity ,MVD)的关系 ,探讨Survivin对口腔鳞癌血管生成的作用及意义。方法 :收集口腔鳞癌标本 42例 ,其中有淋巴结转移者 14例 ,正常口腔黏膜组织 10例 ,用免疫组化染色法探测Survivin和CD3 4 的表达情况并计数微血管密度 (MVD)。结果 :正常口腔黏膜均未见Survivin表达 ,口腔鳞癌中Survivin表达阳性率为 80 .95 % ( 3 4/4 2 )。口腔鳞癌中MVD显著高于正常组织 ,且随病理分化不良而增高 (P <0 .0 5 ) ,有淋巴结转移组MVD显著高于无淋巴结转移组 (P <0 .0 5 ) ,Survivin表达阳性组中MVD明显高于Survivin表达阴性组 (P <0 .0 5 ) ,Survivin的表达与MVD呈正相关 (P <0 .0 5 )。结论 :Survivin在口腔鳞癌组织中的高度表达在口腔鳞癌发生发展中起重要作用 ,并与其血管生成和淋巴结转移有关
OBJECTIVE: To observe the expression of Survivin and its relationship with microvessel density (MVD) in oral squamous cell carcinoma (OSCC) and to investigate the effect and significance of Survivin on angiogenesis in OSCC. Methods: Forty-two patients with oral squamous cell carcinoma were collected, including 14 cases with lymph node metastasis and 10 cases with normal oral mucosa. The expressions of Survivin and CD34 were detected by immunohistochemistry and the microvessel density (MVD) was counted. Results: No expression of Survivin was detected in normal oral mucosa. The positive rate of Survivin in oral squamous cell carcinoma was 80.95% (34/42). The MVD in oral squamous cell carcinoma was significantly higher than that in normal tissues and increased with pathological differentiation (P <0.05). The MVD in lymph node metastasis group was significantly higher than that in non-lymph node metastasis group (P <0.05), and the expression of Survivin MVD in positive group was significantly higher than that in Survivin negative group (P <0.05), Survivin expression was positively correlated with MVD (P <0.05). Conclusion: The high expression of Survivin in oral squamous cell carcinoma plays an important role in the occurrence and development of oral squamous cell carcinoma, and is related to its angiogenesis and lymph node metastasis